Renfiyeni, Yusniwati, Jumsu Trisno and Jamsari


Calli is one of the materials used for genetic transformation. Studies on calli induction in some pepper genotypes were conducted in the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Andalas University, Padang. This study aimed to obtain calli which can be used as material for genetic transformation. We used three genotypes of chili (Kopay, Kampung and Trisula). Explants were derived from first leaf, cotyledon, epycotyl and hypocotyl obtained from 24 days old chili germination. The study found that all the explants were able to produced calli. From 17 combinations of medium, the two combinations that produced 100% calli were MS + BAP + IAA + L2 vitamin and MS + 2,4 D. Mediums that can produce organogenic calli were MS + BAP 4 mg / L, IAA 0.5 mg / L, L2 vitamin ( Pyridoxin hydroklorit 0.5 g / L  and Thiamin hydroklorit 0.1 mg / L). The colour of calli produced were yellowish green and compact. The study also found that structures develop with a globular phase, followed by a heart shape, torpedo, cotyledonary and eventually form buds. While MS + 2.4 D 4 mg / L produced yellowish white calli and crumb, it could not grow organogenic calli.


crop-livestock integration; palm oil by-products technology

Full Text:



Ahmad, I.B., M.N. Hanina, A.M. Mahir, and A. Zaiton, 2002. Transformation of Red Chilli Variety Cilibangi-2 (Capsicum annum L.) with cDNA of Cucumber Mosaic Virus Coat Protein Gene by Direct Uptake. Pakistan Journal of Biological Science 5 (6): 683-687.

Ahmad,I.B.,Y.H.Leong and Y.S.Lee, 1997. Analysis of factors affecting transformation of Capsicum annuum with Agrobacterium. Proceedings of 9th National Biotechnology Seminar: Positioning National R & D in Biotechnology for Global Competitiveness, pp: 131-133.Penang Malaysia 23-26 November 1997

Ashrafuzzaman, M., M.M.Hosain, M.Razi Ismail, M.Shahidul Haque, S.M.Shahidullah, and Shanin-Uz-Zaman, 2009. Regeneration potential of seedling expalnts of chili (Capsicum annuum). African Journal of Biotechnology Vol 8(4), pp 591-596. Feb 2009

Aykas , A.M. 1990. Expectations and limitations of using growth regulators in the engineering of crop cultivation in the collection of conference papers National Agrochemicals , January 20 , 1990. Jatinangor . It 9-17 .

Bhaskaran, S and R.H. Smith, 1990. Cell biology and molacular genetic regeneration in l Courncereal tissue culture, Journal Crop Science 30 :1328-1336

Bosland,P.W. and Votava, E.J. 2000. Peppers : Vegetables and spice capsicums. Crop Production science in Horticulture 12. CAB Internasional Publishing Wallingford, England, U.K p:204

Christopher, T and Rajam M.Y, 1996. Effect of genotype, explants and medium on in vitro regeneration of red pepper. Plant Cell Tissue Organ Cult. 46: 245-250

Dabauza, M., Penal L. 2001. High efficiency organogenesis in sweet pepper (Capsicum annuum L.) tissue from different seedling explants. Plant Growth Regul. 33. 21-229

Diaz I, Moreno R., Power J.B, 1988. Plant regeneration from protoplasts of Capsicum annuum. Plant Cell Rep. 7 : 210-212.

George, E.F and P.D. sherington, 1984. Plant Propagation by Tissue Culture. Handbook and Directory of Comercial Laboratory. Exegheties Ltd. England. 709p

Gunawan , L.W. 1988. Plant Tissue Culture Technique . PAU Biotechnology.IPB. Bogor

Gunay, S. And Rao P.S, 1978. In vitro plant regeneration from hypocotyls and cotyleldon explants of red pepper ( C. annuum) Plant Sci. Lett. 11 : 365-372

Katuuk , J.R.P. 1989. Tissue Culture Technique in plant micropropagation . Ministry of Education and Culture . Directorate General of Higher Education . Educational Institutions Development Project Personnel . Jakarta . 188 p.

Nono-Wondim, R., G. Marchoux, E. Pochard, A. Palloix, and K. Gebre Selassie. 1991. Resistance of pepper lines to movement of Cucumber Mosaic Virus. J. Phytopathol. 137 : 21-32

Prajnanta, F. 1999. Planting Ways Chili in rainy season. Penebar Swadaya. Jakarta.

Sulandari, S. 2004. Characterization biology , serology and DNA fingerprinting analysis of viruses that cause disease yellow leaf curly chili . Dissertation Graduate School of IPB Bogor

Taji, M. Acram, Dood, A., William and Willians R.Ricard. 1992. Plant tsissue culture practise. Botani Departemen and Departemen of Agronomi and Soil Science, University of New England, Armidale. Centre for Biological Population Management Queeslend University, Brisbane.

The Central Bureau of Statitik . 2011. Harvested Area , Production and Productivity Chili , 2009. (09 March 2011) .

Yusniwati, 2008. Drought Tolerant Transgenic Chili strains with P5CS Gene Encoding Key Enzyme Biosistesis Proline: Regeneration and Characterization regenerants. Dissertation Graduate School of Bogor Agricultural University. 119 p

Watimena, G.A. 1991. Plant Biotechnology . Plant Tissue Culture Laboratory team . University Centre of Biotechnology .IPB . Bogor


  • There are currently no refbacks.