Effect of Rice Milling on Their Polyphenol, Protein Content and Antioxidant Activity

Soyema Khatun, Md. Mahi Imam Mollah

Abstract

The phenolic compound is the main source of antioxidants that reduces the incidence of chronic diseases such as heart disease, blood cholesterol, blood pressure, diabetes, etc. This experiment aimed to investigate the effect of the three milling processes of rice (dehusking, auto, and polishing) on polyphenol content with their antioxidant activity and nutritional profile. The polyphenol content was determined according to the Folin Ciocalteu method. Polishing rice contained a maximum phenolic compound (36.28 ± 0.73mg/g of extract) that was statistically different from other milling rice (p<0.05). Polishing rice contained the highest amount of protein (262.69 ± 0.38mg/g extract), whereas auto rice contained the highest amount of free amino acid content (14.06 ± 0.46mg/g extract) and total sugar content (1312.29 ± 2.28mg/g extract). Reducing sugar content was statistically similar to all milling rice. Antioxidant activity was comparatively assessed by ABTS (2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) free radical decolorization assay method. It was investigated that polishing rice showed strong free radical scavenging capacity by ABTS radical. It is concluded that polishing rice can be used as a functional ingredient with high polyphenol and protein content with strong antioxidant activity.

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References

Brand-Williams, W., Cuvelier, M.E., and Berset, C.L.W.T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, Vol. 28, 25-30.

Brouns, F., Hemery, Y., Price, R., Anson, N.M. (2012). Wheat aleurone: Separation, composition, health aspects, and potential food use. Crit. Rev. Food Sci. Nutr. Vol. 52, 553–568.

DuBois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.T., and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry. Vol. 28, 350-356.

FAO. FAOSTAT. (2021). FAO Statistics Division, Rome.

FAO (Food and Agriculture Organisation). (2002). World Agriculture: Towards 2015/2030. Summary Report. FAO, Rome.

Goffman, F.D. and Bergman, C.J. (2004). Rice kernel phenolic content and its relationship with antiradical efficiency. J Sci Food Agr. Vol. 84, 1235-1240.

Hu, C., Zawistowski, J., Ling, W., Kitts, D.D. (2003). Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem. Vol. 51, 5271-5277.

Hu F.B. (2003). Plant-based foods and prevention of cardiovascular disease: an overview. Am J Clin Nutr. Vol. 78, 5445–5515 2.

Hudson, E.A., Dinh, P.A., Kokubun, T., Simmonds, M.S.J., Gescher, A. (2000). Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidem Biomar. Vol. 9, 1163-1170.

Hyun, J.W., Chung, H.S. (2004). Cyanidin and malvidin from Oryza sativa cv. Heugjinjubyeo mediates cytotoxicity against human monocytic leukemia cells by the arrest of the G2/M phase and induction of apoptosis. J Agric Food Chem. Vol. 52, 2213-2217.

Itani, T., Tatemoto, H., Okamoto, M., Fujii, K., Muto, N. (2002). A comparative study on antioxidative activity and polyphenol content of colored kernel rice. J Jpn Soc Food Sci. Vol. 49, 540-543.

Lantin, R. (1999). RICE: Post-harvest Operations. AGSI/FAO.

Ling, W.H., Cheng, Q.X., Ma, J., Wang, T. (2001). Red and black rice decrease atherosclerotic plaque formation and increase antioxidant status in rabbits. J Nutr. Vol. 131, 1421-1426.

Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. Vol. 193, 265-275.

Morimitsu, Y., Kubota, K., Tashiro, T., Hashizume, E., Kamiya, T., Osawa, T. (2002). Inhibitory effect of anthocyanins and colored rice on diabetic cataract formation in the rat lenses. Int Congr Ser. 245, 503-508.

Nelson, N. (1944). A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry. Vol. 153, 375-380.

Orlich M.J., Fraser G.E. (2014). Vegetarian diets in the Adventist Health Study 2: a review of initial published findings. Am J Clin Nutr. Vol. 100, 353S–358S.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M. (1999). Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad Biol Med. Vol. 26:1231-1237.

SAS Institute Inc. (1989). SAS/STAT user's guide, Release 6.03, Ed. Cary, NC.

Setsuro Matsushita, Nobuko Iwami, YukiNitta. (1966). Colorimetric estimation of amino acids and peptides with the Folin phenol reagent. Analytical Biochemistry. Vol. 16 (2), 365-371.

Singleton, V.L., Orthofer, R., and Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin ciocalteu reagent. Methods in Enzymology. Vol. 299, 152-178.

Somogyi, M. (1952). Notes on sugar determination. J. Biol. Chem. Vol. 195(1), 19-23.

Tepe B., Sokmen M., Akpulat H.A., Sokmen A. (2006). Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem. Vol. 95, 200-204.

World Health Organization (WHO) report. (2018). Prevention and Control of Noncommunicable Diseases in the European Region: A Progress Report.

Yawadio, R., Tanimori, S., & Morita, N. (2007). Identification of phenolic compounds isolated from pigmented rice and their aldose reductase inhibitory activities. Food Chemistry. Vol. 101, 1616–1625.

Zhang, M., Guo, B., Zhang, R., Chi, J., We, Z., Xu, Z., Zhang, Y., and Tang, X. (2006). Separation, purification, and identification of antioxidant compositions in black rice. Agric Sci China. Vol. 5, 431-440.

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