Specific RAPD frafments related with sex type in Salacca (Salacca edulis L.)

Ediwirman, Irfan Suliansyah, Gustian and Jamsari


Salacca (Salacca edulis L.) is a tropical plant with a high economic value. The sexes of salacca can be grouped as dioecious, monoecious, dan hermaphrodite. Farmer's success in cultivation is especially determined by the male to female ratio. The present ratio of male and female plants is 1:4. Determining sex based on morphological characteristics is not effective and difficult for young plants (seedlings), also to lysozim marker and chromosome. Marker Assisted Selection is a strategy to determine sex using a marker. Random Amplifed Polymorfism DNA (RAPD) is an amplification technique that uses markers. Of 305 primers tested, 4 primers, OPO-17 produced 297 bp product, OPAP-20 produced 554 bp as the specific fragment for the female and UBC-454 produced 934 bp as the specific fragment for the female and UBC-454 produced 946 bp as male, primer UBC-78 with the size of fragment 562 bp as the specific fragment for the female.


Sex determination; dioecious; RAPD molecular marker; Marker Assisted Selection (MAS)

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Adawy, S. S, J. Jiang, and M. A. M. Atia. 2014. Identification of novel sex-specific PCR-Based Markers to distinguish the genders in Egyptian date palm trees. International Journal Of Agricultural Science And Research (Ijasr) 4, 45-54.

Agrawal V, Sharma K, Gupta S, Kumar R, and Prasad M. 2007. Identification of sex in Simmonsia chinensis (Jojoba) using RAPD markers. Plant Biotechnol. Rep. 1: 207-210.

Banerjee NS, Manoj P, and Das MR. 1999. Male-sex-associated RAPD markers in Piper longum. Current Science, 77: 693-697.

Bouza N, Caujape-Castells J, Gonzalez-Perez MA, and Sosa PA. 2006. Genetic structure of natural populations in the red algae Gelidium canariense (Gelidiales, Rhodophyta) investigated by random amplified polymorphic DNA (RAPD) markers. J. Phycol. 42: 304-311.

Dainimihardja. S. 1994. Lembaran informasi porsea. (5): 29 – 30.

Deputy JC, Ming R, Ma H, Liu Z, Fitch MMM, Wang M, Manshardt R, and Stiles JI. 2002. Molecular markers for sex determination in papaya (Carica papaya L.). Theor. Appl Genet. 106: 107-111.

Doere. P, S. Samantaray, and S. Maiti. 2014. Genomc DNA isolation protocol for Aloe barbadensis Miller. Using leaf gel for genetic charactarization. International Journal of Scientific and Research Publication. 4: p. 1 – 5.

Doyle J. J. and J. L. Doyle. 1987. A rapid DNA isolation procedures for small quantities of fresh leaf tissues. Phytochem Bull. 19, 11-15.

Ehsanpour. A. A, and L. Arab. 2009. Application of RAPD (Random Amplified Polymorphic DNA) marker for sex determination of Pistacia vera L. Journal of Cell and Molecular Research. 1 (2), 68-71

Hadi. P.S, A. Purwanto, dan D. Prajitno. 2002. Identifikasi kromosom dalam penentuan jenis kelamin salak (Salacca zalacca (Gaertner) Voss. Agrosain. 15 (1) Januari 2002. 31 – 46 hal.

Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J. 1986. Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor. Appl. Genet. 61:650-658.

Hormaza J. I., Dollo L. and Polito V. S. 1994. Identification of a RAPD marker linked to sex determination in Pistacia vera using Bulked Segregant Analysis. Theoretical and Applied Genetic 89: 9-13.

Hosseini. F. S, H. S. Hassani, M. J. Arvin, A. Baghizadeh and G. M. Nejad. 2011. Sex determination of jojoba (Simmondsia chinensis cv. Arizona) by Random Amplified Polymorphic DNA (RAPD) molecular markers. African Journal of Biotechnology. 10: 470-474.

Hou W, Fan J, Zhou F, and Zhao S. 2009. RAPD markers related to sex locus in Populus tomentosa. Frontiers Forest. China, 4: 223-226.

Ii, Y., Uragami, A., Uno, Y., Kanechi, M. and Inagaki, N. 2012. RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis. Horticulture Science (Prague) 39, 33-37.

Jamsari A., Nitz I., Reamon-Büttner S.M., and Jung C., 2004. BAC-derived diagnostic markers for sex determina­tion in asparagus. Theoretical and Applied Genetics, 108: 1140–1146.

Jeffreys AJ, Wilson V, and Thein SL. 1985. Hyper variable mini satellite regions in human DNA. Nature. 314: 67-73.

Kafkas, S., Cetiner, S. and R. Perl-Treves. 2001. Development of sex-associated RAPD markers in wild Pistacia species. J. Hort.Sci. and Biotech. 76 (2): 242-246.

Lemos E.G.M, Silva C.L.S. P, and Zaidan HA. 2002. Identification of sex in Carica papaya L. using RAPD markers. Euphytica, 127: 179-184.

Mathew. K.M, S. Jose, Y. S. Rao, U. Gupta, and J. Thomas. 2014. Optimization of genomic DNA extraction from fresh and dry leaves of large cardamon (Amomum subulatum Roxb.) for diversity analysis. Indian Journal of Biotechnology. 13: pp. 221-224.

Michelmore, R.W., I. Paran, and R.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by Bulked Segregant Analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Natl. Acad. Sci. USA, 88: 9829-9832.

Ming R, Wang J, Moore P. H, and Paterson A.H. 2007. Sex chromosomes in flowering plants. Am. J. Bot. 94: 141-150.

Packer, C., Gilbert, D. A., Pusey, A. E. and O´Brien, S. J., 1991. A molecular genetic analysis of kinship and cooperation in African lions. Nature, 351: 562–565.

Parjanto, S. Moeljopawiro, W. T. Artama, dan A. Purwantoro. 2006. Identifikasi penanda RAPD untuk penentuan jenis kelamin tanaman salak (Salacca zalasca Gart Voss.). Berkala Ilmiah Biologi. 5(1): 57-63.

Parrish TL, Koelewijn HP, and van Dijk PJ. 2004. Identification of a male-specific AFLP marker in a functionally dioecious fig, Ficus fulva Reinw. ex Bl. (Moraceae). Sex. Plant Reprod. 17: 17-22.

Pourkazemi, M and S. Razikazemi. 2011. Failure of PCR-RAPD technique to differentiate sex in Mahisefied (Rutilus frisii kutum) from the South Caspian Sea. Caspian J. Env. Sci.. 9(2). pp. 235-242.

Reamon-Büttner S.M., Schondelmaier J., and Jung C., 1998. AFLP markers tightly linked to the sex locus in Asparagus officinalis L. Molecular Breeding, 4: 91–98.

Rohde W. 1996. Inverse sequence-tagged repeat (ISTR) analysis, a novel and universal PCR-based technique for genome analysis in the plant and animal kingdom. Journal of Genetics Breed. 50: 249-261.

Schuiling, D.L. and J.P. Mogea. 1992. Salacca zalacca (Gaertner) Voss. In Verheij, E.W. M. and Coronel, R.E. (eds.). Edible Fruits and Nuts. Plant Resources of South East Asia no. 2. PROSEA. Bogor. Indonesia

Spada A, Caporali E, Marziani G, Portaluppi P, Restivo FM, Tassi F, and Falavigna A. 1998. A genetic map of Asparagus officinallis based on integrated RFLP, RAPD, and AFLP molecular markers. Theor. Appl. Genet. 97: 1083-1089.

Urasaki N., Tokumoto M. Tarora K., Ban Y., Kayano T., Tanaka H., Oku H., Chinen I. and Terauchi R. 2002. A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.) Theoretical. Applied Genetic 104: 281–285.

Vaidya. G, and G.R. Naik. 2014. Molecular identification of sex in Simarouba glauca by RAPD markers for crop improvement strategies. Biotechnology Reports. 4: 56–59

Weising. K, Nybom. H, Wolff. K, and Kahl. G. 2009. DNA fingerprinting in plant: principles, methods, and applications, 2nd end. (CRC. Press, Boca Raton, Florida, USA. Pp. 91-93.

Xu W, Wang B, and Cui K. 2004. RAPD and SCAR marker linked to sex determination in Euccommmia ulmoides Oliv. Euphytica 136: 233-228.


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